α-Latrotoxin |
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| PRODUCT LINE | Natural Products / Antibiotics |
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| PRODUCT FAMILIES | Neurotoxins |
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| Ordering Information |
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| Product Numbers | Format | Size | Unit Price | Quantity | | | ALX-630-027-C040 |
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40 µg | | | | | | |
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| Product Specification |
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| MW: | ~130kDa. |
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| CAS NUMBER: | 65988-34-3 |
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| RTECS: | OE9020000 |
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| SOURCE/HOST: | Isolated from Latrodectus tredecimguttatus. |
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| QUANTITY: | Determined by Lowry and Pierce method. |
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| PURITY: | ≥97% |
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| APPEARANCE: | Lyophilized. |
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| PURITY DETAIL: | Migrates as one distinct band when run on a 5-25% gradient polyacrylamide gel according to the method of Laemmli (Nature 227, 680 (1970)). |
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| RECONSTITUTION: | Reconstitute in 0.5ml of distilled cold water. Stir very gently. Do not shake or vortex. Add 0.5 ml glycerol. Stock solution received contains 50% glycerol and 300nM α-latrotoxin. |
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| QUALITY CONTROL: | Bioassay: Test of the ability to stimulate neurotransmitter release in both Ca2+-free and Ca2+-containing media according to Valtorta. Effective concentration: 100pM-1nM. |
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| SHIPPING: | SHIPPED ON BLUE ICE |
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| LONG TERM STORAGE: | -20°C |
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| USE/STABILITY: | Stock solution is stable for 1 week when stored at +4°C or for at least one year at -20°C. |
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| HAZARD: | TOXIC. |
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| Product Description |
Causes massive neurotransmitter release from a wide variety of central and peripheral synaptic junctions of vertebrates using Ca2+-dependent and Ca2+-independent pathways. A useful pharmacological tool in the studies of synaptic vesicles exocytosis of different neutrotransmitters. |
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| | Product Specific Literature References | Purification from black widow spider venom of a protein factor causing the depletion of synaptic vesicles at neuromuscular junctions: N. Frontali, et al.; J. Cell. Biol. 68, 462 (1976) Abstract
Preparation and properties of a neurotoxin purified from the venom of black widow spider (Latrodectus mactans tredecimguttatus): A. Grasso; Biochim. Biophys. Acta 439, 406 (1976) Abstract
Synaptophysin (p38) at the frog neuromuscular junction: its incorporation into the axolemma and recycling after intense quantal secretion: F. Valtorta, et al.; J. Cell. Biol. 107, 2717 (1988) Abstract
The effect of alpha-latrotoxin on a synaptic connection between identified neurons in the brain of the mollusc Helix pomatia L: O.N. Osipenko, et al.; Toxicon 31, 1123 (1993) Abstract
GTP cleavage by the small GTP-binding protein Rab3A is associated with exocytosis of synaptic vesicles induced by alpha-latrotoxin: B. Stahl, et al.; J. Biol. Chem. 269, 24770 (1994) Full Text; http://www.jbc.org/cgi/reprint/269/40/24770.pdf
Alpha-latrotoxin stimulates glutamate release from cortical astrocytes in cell culture: V. Parpura, et al.; FEBS Lett. 360, 266 (1995) Abstract
Mechanisms of alpha-latrotoxin action: A. W. Henkel & S. Sankaranarayanan; Cell Tissue Res. 296, 229 (1999), (Review) Abstract
alpha-Latrotoxin and its receptors: neurexins and CIRL/latrophilins: T.C. Sudhof; Annu. Rev. Neurosci. 24, 933 (2001), (Review) Abstract
Alpha-latrotoxin induces exocytosis by inhibition of voltage-dependent K+ channels and by stimulation of L-type Ca2+ channels via latrophilin in beta-cells: S. Lajus, et al.; J. Biol. Chem. 281, 5522 (2006) Abstract |
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| RELATED PRODUCT GROUPS |
Signal Transduction > Ion Channels & Transporters / Related Products > Ca2+ Channels > Ca2+ Modulators / Protein Synthesis, Modification & Degradation > Vesicles & Membrane Trafficking > Exocytosis |
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