LB Broth media formulations have been an industry standard for the cultivation of Escherichia coli since the 1950's. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. The three formulations differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions.
|Long Term Storage:||Ambient|
|Background / Technical Information:||Method of Preparation|
1. Dissolve 15.5, 20 and 25 grams, LB-Luria, LB-Lennox, LB-Miller, respectively, in 1 liter of deionized water. For solid medium, add 15 g bacteriological agar.
2. Autoclave at 121°C for 15 min. Store liquid medium at 4°C.
3. For solid medium, allow to cool to 45°C. Supplement with antibiotic as needed. Pour about 30 ml per 100 mm petri dish. Allow to cure overnight. Store at 4°C in plastic bags.
Note: To promote faster growth, the medium can be supplemented with 10 ml 10% sterile glucose or 40 ml 10% sterile glycerol.