Hyper Broth™ is a proprietary medium formulation for improved expression of recombinant proteins in E. coli. It is made without animal products and is certified "animal product free" (APF). The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels. This medium produces the highest level of biomass production. The medium is buffered at pH 7.5±0.2. This medium has the highest buffering capacity of the expression media set, which permits high glucose levels. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 4 to 5 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Plasmid copy numbers are higher than for LB, which can facilitate protein expression. This medium is suitable as the base for fermentation cultures and is excellent for feedbatch applications. Biomass yields will depend on the process and the strain employed.
|Long Term Storage:||Ambient|
|Background / Technical Information:||Method of Preparation: |
1. Dissolve 44.5 grams in 950 ml deionized water.
2. Dispense as required.
3. Autoclave at 121°C for 15 min.
4. Aseptically add 50 ml of Glucose Nutrient Mix. Mix well.