The PDQ Protease Assay is a colorimetric assay used to detect protease activity. The proprietary substrate is susceptible to a wide range of proteases, e.g., collagenase, proteinase K, papain, pepsin, bromelin, ficin, trypsin and chymotrypsin. This substrate is a cross-linked matrix containing protein and a dye-protein conjugate. The matrix is supplied in ready-to-use vials. Test samples containing putative protease activity are aliquoted into the vials which are then incubated at an appropriate temperature.
|Short Term Storage:||+4°C|
|Long Term Storage:||+4°C|
|Kit/Set Contains:||48 vials containing 200µl of pre-made matrix|
|Background / Technical Information:||Buffers that can be used to prepare test samples may range in pH from 4 to 10.|
Materials not provided but required:
- 0.2N NaOH
- Spectrophotometer for reading absorbance at 450 nm