All Axxora Brands
Axxora Brands available in the selected country
Join Axxora
Corporate Profile
Sites
You are logged on as Guest.:  LoginRegister(Empty)
Change country
  United States
  • Home
  • Product Detail
New Products
Brands
Distributors
Support
About

Rp-8-AEA-cAMPS-Agarose

Immobilized cAMP Antagonist
 
BLG-A007-06 0.6 ml 257.00 USD
 
BLG-A007-25 2.5 ml 801.00 USD
 
BLG-A007-60 6 ml 1,559.00 USD
 
The metabolically stable protein kinase A inhibitor Rp-cAMPS immobilized on agarose by an aminoethylamino spacer attached to position 8 of the ligand. Free Rp-cAMPS binds to the protein kinase A holoenzyme but prevents dissociation to free catalytic and regulatory subunits. In addition, it is not hydrolized by mammalian phosphodiesterases.The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinase A (presumably as its holoenzyme), phosphodiesterases and others.
more
This ligand is also available with a longer spacer arm (Rp-8-AHA-cAMPS-Agarose Prod. No. BLG-A012)).
less
PRODUCT PROPERTIES
Alternative Name:8-(2-Aminoethyl)aminoadenosine-3',5'-cyclic monophosphorothioate, Rp-isomer; immobilized on agarose gel
Formulation:Liquid. 0.05M Na2HPO4 buffer (pH7), containing 0.1% sodium azide.
Long Term Storage:+4°C
Miscellaneous/General:After equilibration with about 10 column volumes of starting buffer the affinity column is loaded with the protein solution at 50-100µl/min. In order to elute unspecifically bound nucleotide-dependent proteins the column can be washed, e.g. with 1mM 5'-AMP. Elution of the target protein is performed by a cyclic nucleotide gradient up to 1mM. For elution of phosphodiesterases it could be advisable to use the highly resistant Sp-cAMPS or the completely stable Rp-cAMPS. For protein kinase holoenzyme elution the gradient should be made with Rp-cAMPS.

Suitable buffer systems for your special application have to be tested but phosphate should be not optimal since one essential affinity interaction of cyclic nucleotides towards their target receptors is the cyclic phosphate. Regeneration can be achieved by a combination of up to 100 mM cAMP and buffer salts or 8 M urea.
Background / Technical Information:For the Original Manufacturer's data sheet please click here.
Formula:
BLG-A007

TECHNICAL DOCUMENTS

Print as PDF

RELATED PRODUCTS

No related products available

PRODUCT SUPPORT

Technical Service
Bulk Quantity Quote
Customer Service

CATALOG SECTION

cAMP-dependent Protein Kinase [PKA]
cAMP-dependent Protein Kinase [PKA]
cAMP-dependent Protein Kinase [PKA]
Adenosine Derivatives
Adenosine Derivatives
Adenosine Derivatives
Adenosine Derivatives
cAMP Derivatives
Cyclic Nucleotides
Cyclic Nucleotides
Cyclic Nucleotides
Cyclic Nucleotides
Bone Metabolism
Cancer
Cell Cycle
Cell Death / Apoptosis / Autophagy / Necrosis
Chemokines & Cytokines
Cytoskeleton
DNA & Protein Labeling
DNA Regulation / Transcription
Drug Discovery
Dyes / Stains / Fluorescent Probes
Epigenetics
Immunology
Inflammation
Molecular Biology / Genomics
Natural Products / Antibiotics
Neurobiology
Nitric Oxide Pathway
Obesity & Adipokines
Oxidative Stress
Proteasome & Related Complexes
Protein Expression / Modification
Signal Transduction
Stress & Heat Shock Proteins
Ubiquitin & Ubl Signaling
Other Products
another CS2 website
©2018 Axxora, LLC.,
  • Privacy
  • Disclaimer
  • Terms
  • Legal
  • Sitemap
  • Feedback