All Axxora Brands
Axxora Brands available in the selected country
Join Axxora
Corporate Profile
Sites
You are logged on as Guest.:  LoginRegister(Empty)
Change country
  United States
  • Home
  • Product Detail
New Products
Brands
Distributors
Support
About

Rp-2'-AHC-cGMPS-Agarose

PDE-resistant Rp-cGMPS immobilized on agarose gel
 
BLG-A051-06 0.6 ml 245.00 USD
 
BLG-A051-25 2.5 ml 766.00 USD
 
BLG-A051-60 6 ml 1,490.00 USD
 
The PDE-stable protein kinase G (PKG) inhibitor Rp-cGMPS immobilized on agarose by an aminohexylcarbamoyl spacer attached to the ribose 2' position of the ligand. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those which tolerate modification of the 2' ribose hydroxyl group, such as some phosphodiesterases. This type of gel is also available with immobilized PDE-resistant Sp-cGMPS (Sp-2'-AHC-cGMPS-Agarose (Prod. No. BLG-A052)) and with normal cGMP (2'-AHC-cGMP-Agarose (Prod. No. BLG-A059)).
PRODUCT PROPERTIES
Alternative Name:2'-(6-Aminohexylcarbamoyl)guanosine-3',5'-cyclic monophosphorothioate, Rp-isomer, immobilized on agarose gel
Long Term Storage:+4°C
Miscellaneous/General:

Chromatography: After equilibration with about 10 column volumes of starting buffer the affinity column is loaded with the protein solution at 50 - 100 µl/min. In order to elute other nucleotide-dependent proteins unspecifically bound, the column is washed, e.g. with 1mM  5'- GMP.
Elution of the target protein is performed by a cyclic nucleotide gradient. For elution of phosphodiesterases it could be advisable to use the highly hydrolysis-resistant phosphorothioate-modified analogs or PDE inhibitors.
Suitable buffer systems for your special application have to be tested, but phosphate should be not optimal since one essential affinity interaction of cyclic nucleotides towards their target receptors is the cyclic phosphate.
Regeneration can be achieved by a combination of up to 100 mM cGMP and buffer salts or 8 M urea.

Background / Technical Information:For the Original Manufacturer's data sheet please click here.
Formula:
BLG-A051

TECHNICAL DOCUMENTS

Print as PDF

RELATED PRODUCTS

No related products available

PRODUCT SUPPORT

Technical Service
Bulk Quantity Quote
Customer Service

CATALOG SECTION

Cyclic Nucleotides
Cyclic Nucleotides
Cyclic Nucleotides
Cyclic Nucleotides
Guanosine Derivatives
Guanosine Derivatives
Guanosine Derivatives
Guanosine Derivatives
cGMP Derivatives

General Literature References

Binding of adenosine 3',5'-monophosphate dependent protein kinase regulatory subunit to immobilized cyclic nucleotide derivatives: W.L. Dills, Jr., et al.; Biochemistry 15, 3724 (1976), Abstract;
Purification of rabbit skeletal muscle protein kinase regulatory subunit using cyclic adenosine-3':5'-monophosphate affinity chromatography: W.L. Dills, Jr., et al.; BBRC 62, 70 (1975), Abstract;
Bone Metabolism
Cancer
Cell Cycle
Cell Death / Apoptosis / Autophagy / Necrosis
Chemokines & Cytokines
Cytoskeleton
DNA & Protein Labeling
DNA Regulation / Transcription
Drug Discovery
Dyes / Stains / Fluorescent Probes
Epigenetics
Immunology
Inflammation
Molecular Biology / Genomics
Natural Products / Antibiotics
Neurobiology
Nitric Oxide Pathway
Obesity & Adipokines
Oxidative Stress
Proteasome & Related Complexes
Protein Expression / Modification
Signal Transduction
Stress & Heat Shock Proteins
Ubiquitin & Ubl Signaling
Other Products
another CS2 website
©2018 Axxora, LLC.,
  • Privacy
  • Disclaimer
  • Terms
  • Legal
  • Sitemap
  • Feedback