Analogue of the natural signal molecule cyclic AMP in which the hydrogen in position 8 of the heterocyclic nucleobase is replaced by the lipophilic 4-hydroxyphenylthio moiety. In addition, the ribose 2'- hydroxy group has been methylated.
Potent stimulator of exchange factors directly activated by cAMP (Epac or cAMP-GEF), a newly discovered receptor for cyclic AMP. Since a free 2'-ribose hydroxyl group in cyclic AMP is essential for stimulation of cAMP-dependent protein kinase (PKA), the methylated structure of 8-pHPT-2'-O-Me-cAMP is an extremely poor PKA activator and allows for specific discrimination between both signalling pathways.
Its high lipophilicity ( > dibutyryl-cAMP), allows for good membrane permeability in most biosystems, and its increased resistance towards phosphodiesterases prevents from rapid hydrolysis.
|Alternative Name:||8-pHPT-2'-O-Me-cAMP . Na, 8-(4-Hydroxyphenylthio)-2'-O-methyl-cAMP . Na|
|Formula:||C17H17N5O7PS . Na|
|MW:||466.4 . 23.0|
|Appearance:||Lyophilized or crystallized sodium salt.|
|Solubility:||8-pHPT-2'-O-Me-cAMP has limited solubility in water or buffers, however at least a 100 mM aqueous can be achieved. Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.|
|Long Term Storage:||-20°C|
|Handling:||Protect from light.|
|Miscellaneous/General:||Legal information: the reagent is subject to patent application. It is sold under limited, non transferable licence for research purposes only, to the exclusion of any commercial use.|
|Background / Technical Information:||For the Original Manufacturer's Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer's Data Sheet.
|Manufacturer:||manufactured by Biolog Life Science Institute|
Product Literature References
cAMP analog mapping of Epac1 and cAMP-kinase. Discriminating analogs demonstrate that Epac and cAMP-kinase act synergistically to promote PC-12 cell neurite extension
: A.E. Christensen, et al.; J. Biol. Chem. in press
, (2003), Abstract
; Full Text