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Caspase-3 Colorimetric Assay Kit

Convenient, Highly-Sensitive Assay
 
BV-K106-25 25 assays 145.00 USD
 
BV-K106-100 100 assays 365.00 USD
 
BV-K106-200 200 assays 535.00 USD
 
BV-K106-400 400 assays 835.00 USD
 
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
PRODUCT PROPERTIES
Handling:Protect from light.
Long Term Storage:-20°C
Background / Technical Information:For the Original Manufacturer's data sheet please click here.

TECHNICAL DOCUMENTS

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