Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.
| PRODUCT PROPERTIES |
| Application Notes: | | For the quantification of DEVD-dependent caspase activity by photometry. |
| Handling: | | Protect from light. |
| Long Term Storage: | | -20°C |
| Background / Technical Information: | | For the Original Manufacturer's data sheet please click here. |
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United States
