Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-8 Fluorometric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-8 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
|Application Notes:||For the quantification of IETD-dependent caspase activity by fluorometry.|
|Handling:||Protect from light.|
|Long Term Storage:||-20°C|
|Background / Technical Information:||For the Original Manufacturer's Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer's Data Sheet.