Glutathione Assay Kit (GSH, GSSG & Total)

In the assay, OPA (o-phthalaldehyde), which has a very low fluorescence background, reacts only with GSH (not GSSG), to generate strong fluorescence, so that GSH can be specifically quantified. By adding a Reducing Agent Mix that converts GSSG to GSH, total glutathione can then be determined.
To measure GSSG specifically, a GSH Quencher is added to specifically quench GSH, preventing it from reacting with OPA (while GSSG is unaffected). Reducing Agent Mix is then added to destroy the excess amount of GSH quencher and to convert GSSG to GSH. Thus, GSSG level can be specifically quantified.
The kit also provides unique procedure and buffer formula to eliminate protein thiol interference and to stabilize GSH and GSSG in solutions. The assay is easy to perform and detects 2-400ng/μl of GSH, GSSG or total glutathione.