The evoglow yeast kit contains shuttle plasmids for expression of the respective fluorescent reporter proteins in yeast strains like S. cerevisiae or C. albicans. In pGlow-GalXN-Yt plasmids, the genes encoding for the reporter proteins are under control of the strong Gal1-promoter, which allows repression of gene expression in the presence of glucose. In pGlow-ActXN-Yt constructs, evoglow-Yt is under control of the constitutively and strongly expressed Act1-promoter.
|Quantity:||20 μg each plasmid|
|Long Term Storage:||-20°C|
|Background / Technical Information:||For the Manual please click here.|
|Formulation:||Liquid. In 10mM Tris-HCl buffer, pH 8.5.||