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Propidium Iodide (ultra pure)

DNA dye
 
ENZ-52403 100 mg 156.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-400-043

Propidium iodide (PI) belongs to the same chemical class as ethidium bromide. As with ethidium bromide, its fluorescence is enhanced 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted 30-40 nm and the fluorescence emission maximum blueshifted ~15 nm. PI also binds to RNA as does DAPI and acridine orange. PI is membrane impermeant and is commonly used for identifying dead cells in a mixed population of cells and as a counter-stain in multicolor fluorescent techniques.
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It can also be used to differentiate necrotic, apoptotic and viable cells. The dye is suitable for fluorescence microscopy, flow cytometry and fluorometry applications. Wavelength Maxima: Excitation 535nm, Emission 617nm
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PRODUCT PROPERTIES
Formula:C27H34I2N4
MW:668.4
CAS:25535-16-4
Purity:≥95% (HPLC)
Appearance:Dark red solid.
Solubility:Soluble in DMSO.
Long Term Storage:-20°C
Use/Stability:Stable for at least one year after receipt when stored as recommended.
Handling:Protect from light. Keep cool and dry.
Background / Technical Information:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
Formula:
enz-52403

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CATALOG SECTION

Apoptosis & Necrosis Detection Othe...
CELLestialâ„¢ Fluorescent Probe...
Nuclear / DNA Staining
DNA Intercalators & Crosslinkers

RELATED LITERATURE

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Product Literature References

Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells: N. Pizato, et al.; Sci. Rep. 8, 1952 (2018), Abstract;
The novel protective role of P27 in MLN4924-treated gastric cancer cells: Q. Zhang, et al.; Cell. Death Dis. 6, e1867 (2015), Application(s): Viability staining, Abstract; Full Text
High-throughput cytotoxicity screening by propidium iodide staining: B.S. Edwards, et al.; Curr. Protoc. Cytom. Chapter 9, Unit9 24 (2007), Abstract;
Bone Metabolism
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