pTagCFP-N Expression Vector is a eukaryotic (mammalian) expression vector encoding cyan fluorescent protein TagCFP.
It is designed to generate fusions to the TagCFP N-terminus for expression, localization and cellular dynamics studies or to express TagCFP in eukaryotic (mammalian) cells. pTagCFP-N Expression Vector carries a synthetic version of the TagCFP gene with a humanized codon usage, i.e. optimized for high expression in mammalian cells.
The pTagCFP-N Expression Vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells, pUC origin of replication for propagation in E.
|Application Notes:||Generation of TagCFP-tagged fusions:|
A localization signal or a gene of interest should be cloned into the vector MCS. It will be expressed as a fusion to TagCFP N-terminus when inserted in the same reading frame as TagCFP and no in-frame stop codons are present. TagCFP-tagged fusions retain fluorescent properties of the native protein allowing fusion localization in vivo. Unmodified pTagCFP-N vector will express TagCFP when transfected into eukaryotic (mammalian) cells.
Expression in Mammalian Cells:
pTagCFP-N can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of TagCFP or TagCFP-tagged fusions in many cell types. If required, stable transformants can be selected using G418.
Propagation in E. coli:
· Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue.
· Selectable marker: plasmid confers resistance to kanamycin (30 µg/ml) to E. coli hosts.
· E. coli replication origin: pUC
· copy number: ~500
· plasmid incompatibility group: pMB1/ColE1
|Long Term Storage:||-20°C|
|Background / Technical Information:||For the Original Manufacturer’s Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.