Gateway® TagBFP-AS-C entry clone is a vector containing blue fluorescent protein TagBFP gene variant with codon usage optimized for high expression in Arabidopsis and Saccharomyces. TagBFP coding sequence is flanked by attL1 and attL2 sites allowing easy site-specific recombination. The Invitrogen Gateway® Technology provides a rapid and highly efficient way to transfer the TagBFP gene into a number of Gateway® destination vectors for expression in different experimental systems.
|Application Notes:||Generation of TagBFP-tagged fusions |
A localization signal or a gene of interest can be cloned into MCS of the vector both before and after site-specific recombination with a destination vector. It will be expressed as a fusion to the TagBFP N-terminus when inserted in the same reading frame as TagBFP and no in-frame stop codons are present.
Alternatively, TagBFP gene can be fused to the 3
|Long Term Storage:||-20°C|
|Background / Technical Information:|
For the Original Manufacturer’s Data Sheet please click here.
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.
Product Literature References
An analysis of 5’-noncoding sequences from 699 vertebrate messenger RNAs
: M. Kozak; Nucleic Acids Res. 15
, 8125 (1987), Abstract