pKindling-Red-N vector is an eukaryotic (mammalian) expression vector encoding photoactivatable Kindling Red Fluorescent Protein (KFP-Red). pKindling-Red-N is designed to express KFP-Red in eukaryotic (mammalian) cells for cell movement tracking and to generate KFP-Red-tagged fusions for protein travel studies.
pKindling-Red-N vector contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication and neomycin resistance (Neor) gene for selection in eukaryotic cells.
|Application Notes:||Generation of KFP-Red-fusion proteins|
A localization signal (or a gene of interest) should be inserted into Multiple Cloning Site (MCS) of the vector. It will be expressed as a fusion to KFP-Red N-terminus when inserted in the same reading frame as KFP-Red and no intervening stop codons are present. The inserted sequence should contain an initiating ATG codon. Usually, KFP-Red fusion proteins retain the kindling properties of the native KFP-Red protein.
Note: KFP-Red is a tetramer. This restricts its wide use as a fusion tag for cellular proteins.
Expression in Mammalian Cells
pKindling-Red-N can be transfected into mammalian cells by any known transfection method. If required, stable transformants can be selected.
Propagation in E. coli
· Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue.
· Selectable marker: plasmid confers resistance to kanamycin (30 μg/ml) to E. coli hosts.
· E. coli replication origin: pUC
· Copy number: ~500
· Plasmid incompatibility group: pMB1/ColE1
|Long Term Storage:||-20°C|
|Background / Technical Information:||For the Original Manufacturer’s Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.