KFP-Red is a photoactivatable GFP-like protein, generated by site-directed and random mutagenesis of Anemonia sulcata chromoprotein.
KFP-Red switches from a nonfluorescent to a bright red fluorescent form (with excitation maximum at 580 nm and emission maximum at 600 nm) under the exposure to intense green light irradiation at 530-560 nm. A green light laser does not damage cells and tissues. Activated KFP-Red can be easily detected because its emission spectrum is beyond the region of cell autofluorescence
|Alternative Name:||Kindling-Red Fluorescent Protein (recombinant)|
|Source:||Produced in E.coli. |
|Application Notes:||Control reagent for KFP-Red expression using the Kindling-Red in vivo tracking vectors (Prod. No. EVN-FP301, EVN-FP302, EVN-FP401).|
Monitoring cell and subcellular structure movements in living tissues.
Unique tool to study morphogenesis, inflammation, metastasis, to investigate mitochondria role in apoptosis, vesicular and axonal transport.
|Long Term Storage:||+4°C|
|Background / Technical Information:||For the Original ManufacturerÂ’s Data Sheet please click here.|
Our product description may differ slightly from the Original ManufacturerÂ’s Data Sheet.
Product Literature References
Chromophore environment provides clue to "kindling fluorescent protein" riddle
: D.M. Chudakov, et al.; J. Biol. Chem. 278
, 7215 (2003), Abstract
Kindling fluorescent proteins for precise in vivo photolabeling
: D.M. Chudakov, et al.; Nat. Biotechnol. 21
, 191 (2003), Abstract
Natural animal coloration can Be determined by a nonfluorescent green fluorescent protein homolog
: K.A. Lukyanov, et al.; J. Biol. Chem. 275
, 25879 (2000), Abstract