The pTagRFP-H2B Expression Vector is a mammalian expression vector encoding human histone H2B fused to the TagRFP N-terminus.The vector can be used for fluorescent labeling of histone H2B in living cells. The reporter codon usage is optimized for high expression in mammalian cells (humanized). The vector can be used as a source of TagRFP-H2B hybrid sequence. The vector backbone contains unique restriction sites that permit its excision and further insertion into expression vector of choice.
|Application Notes:||Expression in mammalian cells |
pTagRFP-H2B Expression Vector can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of the TagRFP-H2B fusion in eukaryotic cells. If required, stable transformants can be selected using G418.
Propagation in E. coli
Suitable host strains for propagation in E. coli include DH5alpha, HB101, XL1-Blue, and other general purpose strains. Plasmid incompatibility group is pMB1/ColE1. The vector confers resistance to kanamycin (30μg/ml) to E. coli hosts. Copy number in E. coli is about 500.
|Long Term Storage:||-20°C|
|Background / Technical Information:||Location of Features: |
PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Histone H2B: 657-1034
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1919-1924 & 1948-1953
mRNA 3’ ends: 1957 & 1969
f1 single-strand DNA origin: 2016-2471
Bacterial promoter for expression of Kanr gene
-35 region: 2533-2538
-10 region: 2556-2561
Transcription start point: 2568
SV40 origin of replication: 2812-2947
SV40 early promoter
Enhancer (72-bp tandem repeats): 2645-2716 & 2717-2788
21-bp repeats: 2792-2812, 2813-2833 & 2835-2855
Early promoter element: 2868-2874
Major transcription start points: 2864, 2902, 2908 & 2913
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2996-2998
Stop codon: 3788-3790
G->A mutation to remove Pst I site: 3178
C->A (Arg to Ser) mutation to remove BssH II site: 3524
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4026-4031 & 4039-4044
pUC plasmid replication origin: 4375-5018
For the Original Manufacturer’s Data Sheet please click here.
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.