TurboFP635 (scientific name Katushka) is a novel far-red mutant of the red fluorescent protein derived from sea anemone Entacmaea quadricolor. Possessing excitation/emission maxima at 588/635nm, TurboFP635 is 7 to 10-fold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation and a high pH- and photo-stability. These unique characteristics make TurboFP635 the protein of choice for visualization within living tissues and dual-color high-throughput assays.
The pTurboFP635-C Expression Vector is a C-terminal mammalian expression vector encoding the far-red fluorescent protein TurboFP635.
|Application Notes:||Generation of TurboFP635-fusion proteins |
A localization signal (or a gene of interest) should be cloned into the vector MCS. It will be expressed as a fusion to the TurboFP635 C-terminus when inserted in the same reading frame as TurboFP635 and no in-frame stop codons are present. The fusions retain spectral properties of the native fluorescent protein allowing fusion localization in vivo.
Expression in mammalian cells
The TurboFP635 -C Expression Vector can be transfected into mammalian cells by any known transfection method. The CMV promoter provides strong, constitutive expression of TurboFP635 or TurboFP635 -tagged fusions in many cell types. If required, stable transformants can be selected using G418.
Propagation in E. coli
Suitable host strains for propagation in E. coli include DH5alpha, HB101, XL1-Blue, and other general purpose strains. Plasmid incompatibility group is pMB1/ColE1. The vector confers resistance to kanamycin (30mg/ml) to E. coli hosts. Copy number in E. coli is about 500.
|Long Term Storage:||-20°C|
|Background / Technical Information:||For the Original Manufacturer’s Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.
Product Literature References
Bright far-red fluorescent protein for whole-body imaging
: D. Shcherbo, et al.; Nat. Methods 4
, 741 (2007), Abstract