pHyPer-Cyto is an eukaryotic (mammalian) expression vector encoding a fluorescent sensor for intracellular hydrogen peroxide HyPer. The vector allows to express HyPer in cytoplasm of eukaryotic (mammalian) cells. The vector can also be used as a source of HyPer coding sequence. Flanking restriction sites are convenient for excision of HyPer sequence and its further insertion into other expression vectors of choice. Alternatively, HyPer coding sequence can be amplified by PCR.
pHyPer-Cyto vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells, pUC origin of replication for propagation in E.
|Application Notes:||Expression in Mammalian Cells |
pHyPer-Cyto vector can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of pHyPer in many cell types. If required, stable transformants can be selected using G418.
Propagation in E. coli
· Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue.
· Selectable marker: plasmid confers resistance to kanamycin (30 μg/ml) to E. coli hosts.
· E. coli replication origin: pUC
· Copy number: ~500
· Plasmid incompatibility group: pMB1/ColE1
|Long Term Storage:||-20°C|
|Background / Technical Information:||For the Original Manufacturer’s Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.