Distinct MAP kinase family member. MAP kinases are important components of signal transduction pathways involved in regulating numerous cellular processes including cell growth, division and differentiation. MAP kinases undergo dual phosphorylation on specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. This dual phosphorylation is catalyzed by MEK in vivo and in vitro. Furthermore, in vitro, these sites undergo phosphorylation following incubation of purified MAPK with ATP.
|Alternative Name:||MBPK-2 (bovine)|
|Source:||Isolated from bovine kidney.|
|Formulation:||Liquid. In 50mM TRIS-HCl, pH 7.0, containing 14mM β-mercaptoethanol, 1mM benzamidine, 0.1mM PMSF, 1mM EDTA and 50% glycerol.|
|Activity:||~1’100 units/mg with myelin basic protein (MBP) (Prod. No. GLO-126) as substrate. One unit is the amount of the enzyme that incorporates 1nmol of phosphate into MBP per min.|
|Long Term Storage:||-80°C|
|Handling:||Avoid freeze/thaw cycles.||
: SDS-PAGE pattern of purified preparations of MBPK-1 (Prod. No. GLO-113
) (lane 1) and MBPK-2 (Prod. No. GLO-114
) (lane 2). For MBPK assay kit, see Prod. No. GLO-113-01
. Reproduced with permission from GloboZymes.
Product Literature References
Phosphorylation and activation of protamine kinase by two forms of a myelin basic protein kinase from extracts of bovine kidney cortex
: S.A. Reddy, et al.; J. Biol. Chem. 268
, 15298 (1993), Abstract