The FAM FLICA™ Caspase Detection Assay Kits label active caspases in living cells undergoing apoptosis.
|Quantity:||4 vials of 25 tests.|
|Kit/Set Contains:||- FAM-VAD-FMK FLICA™ lyophilized reagent|
- 10X Wash Buffer
- Propidium Iodide (Prod. No. ALX-400-043).
- Hoechst 33342 stain (Prod. No. ALX-620-050).
|Background / Technical Information:|
For a smaller kit size please see under ICT-91.
For more details see Background Information datasheet and manual.
In Fig. 4 one can clearly distinguish one apoptotic oligodendrocyte from many non-apoptotic cells; the apoptotic cell fluoresces green after labeling with FAM-FLICA™. Apoptotic neurons were also well distinguished from non-apoptotic cells, as seen in Fig. 5. Here FAM-FLICA™ (Prod. No. ICT-92) did reveal that exposure to H2O2 induces apoptosis in rat brain cortical cells, and that continued exposure induces more caspases activity. For the pictures please click here.
Product Literature References
Interactions of fluorochrome-labeled caspase inhibitors with apoptotic cells: a caution in data interpretation
: P. Pozarowski, et al.; Cytometry 55A
, 50 (2003), Abstract
Detection of caspase activation in situ by fluorochrome-labeled caspase inhibitors
: P.A. Amstad, et al.; Biotechniques 31
, 608 (2001), Abstract
Detection of caspases activation by fluorochrome-labeled inhibitors: Multiparameter analysis by laser scanning cytometry
: P. Smolewski, et al.; Cytometry 44
, 73 (2001), Abstract
Stathmo-apoptosis: arresting apoptosis by fluorochrome-labeled inhibitor of caspases
: P. Smolewski, et al.; Int. J. Oncol. 19
, 657 (2001), Abstract
Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation
: E. Bedner, et al.; Exp. Cell Res. 259
, 308 (2000), Abstract
An in situ marker for the detection of activated caspases
: P.A. Amstad, et al.; American Biotechnology Laboratory 18
, 52 (2000), Full Text