The LEXSYcon2 Expression Kit is the system of choice for most proteins. Dependent upon cloning strategy target proteins are either steadily expressed in the cytoplasm of the host or secreted into the growth medium. Cytosolically expressed proteins can be isolated from easily disrupted cells, whereas secreted proteins are efficiently isolated from the medium by one-step affinity chromatography. We recommend the standard kit with the vector pLEXSY-sat2 for selection of LEXSY P10 expression strains with the antibiotic LEXSY NTC.
|Long Term Storage:||-80°C|
LEXSY host P10: The kit contains 3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host P10
pLEXSY-2 expression vector pLEXSY-neo2: 5 µg in 10 mM TrisHCl pH 8.0
Primers for diagnostic PCRs and sequencing vector-specific primers:
neo forward primer A1432, for pLEXSY-neo2
Insert sequencing forward primer P1442
Insert sequencing reverse primer A264
5`utr (aprt) reverse primer A1715
ssu forward primer F3001
ssu reverse primer F3002
Ingredients for 1 liter of culture medium: LEXSY BHI, Powder
LEXSY Neo for pLEXSY-neo2, ready-to-use 1000x stock solution, 50 mg/ml
Hemin, Stock solution, 500x (0.25% solution of porcine Hemin in 50% Triethanolamine)
Pen-Strep, Stock solution, 200x
|Background / Technical Information:|
Contains vector pLEXSY-neo2 (encoding aminoglucoside phosphotransferase).
For the Manual please click here.
LEXSY: Eukaryotic Protein Expression System