Ready-to-use mixture of thermostable DNA polymerase, dNTPs, reaction buffer, stabilizer and loading dye. This reduces any mistakes and contaminations, and provides high reproducibility with various and large numbers of samples. Containing unique reaction buffer and stabilizing agents, it shows a high specificity and stability over to room temperature for at least one month.
- Ready-to-use mix for routine PCR
- Reduced risk of contamination and improved reproducibility
- Significantly reduced PCR preparation time
|Application Notes:||- PCR with high reproducibility|
- High-throughput PCR
- Amplification of genomic, viral and plasmid DNA
|Short Term Storage:||+4°C|
|Long Term Storage:||-20°C|
|Kit/Set Contains:||Concentrated master mix. The addition of template and primers results in 20µl or 50µl containing:|
Thermostable DNA polymerase (from Thermus aquaticus) (1U for 20µl; 2.5U for 50µl)
Stabilizer and tracking dye
|Background / Technical Information:||Protocol|
Prepare PCR premix and add template DNA (5-50ng for 20µl rxn; 10-100ng for 50µl rxn) and primers (5-10pmol for 20µl rxn; 10-30pmol for 50µl rxn) and add water to make a final volume of 20µl or 50µl.