LandMark™ broad range prestained protein marker (dual color) is a dual color prestained size marker. It is a mixture of 10 recombinant purified and prestained polypeptides whose molecular weights are well-adjusted ranging from 7’000 to 240’000Da. It is provided preblended in a ready-to-use formula and no reconstitution or further dilution is necessary before use. A blue and pink chromophore is covalently bound to proteins, and 10 prestained proteins are visible during electrophoresis or electrophoretic transfer from the gel to a membrane.
|Formulation:||Liquid. In 62.5mM TRIS-HCl, pH 6.8, containing 15% glycerol, 1% SDS and 1% β-mercaptoethanol.|
|Long Term Storage:||-20°C|
|Background / Technical Information:|
- Recommended loading volume: 5µl/lane
- Typical number of lanes: 100
- Number of bands: 10
- Range (kDa): 7-240
Because coupling of chromophore to the proteins affects their apparent molecular weights in SDS-PAGE, unstained protein standard should be used for accuracy >95%.
15% SDS-PAGE, 5µl/lane