The SpinClean™ Glutathione Excellose® kit provides fast and one step method for small-scale purification of GST fusion protein. It uses ready-to-use Glutathione Excellose® spin column containing and centrifugation for rapid processing of multiple samples.
| PRODUCT PROPERTIES |
| Long Term Storage: | | +4°C |
| Contents: | | Glutathione Excellose®
Spin column
Collection tube
Stock solutions |
| Background / Technical Information: | | Protocol
1. Vortex the glutathione excellose tube and pipet 0.5ml of the slurry to spin column, add 0.5ml of equilibration buffer in the spin column, and centrifuge at 700 x g (approx. 2’000rpm) for 2min.
2. Discard the eluate, load up to 0.5ml of cell lysate, centrifuge and collect the flow-through fraction.
3. Add 0.5ml of washing buffer, centrifuge and collect the wash fraction.
4. Repeat step 3, if necessary.
5. Add 0.2ml of elution buffer and centrifuge.
6. Collect the eluate. |
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FIGURES
Figure: Principle of SpinClean™ Glutathione Excellose ® Kit (Prod. No. MBI-21020 (20 preparations) and MBI-21050 (50 preparations)).
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United States
