SpinClean™ MBP (maltose binding protein) Excellose® spin kit provides a fast and one step method for small-scale purification of MBP fusion protein. It uses ready-to-use MBP Excellose® spin column and centrifugation for rapid processing of multiple samples.
| PRODUCT PROPERTIES |
| Long Term Storage: | | +4°C |
| Kit/Set Contains: | | MBP Excellose® spin column
Collection tube
Stock solutions |
| Background / Technical Information: | | Protocol
1. Vortex the MBP Excellose® tube and pipet 0.5ml of the slurry to spin column, add 0.5ml of equilibration buffer in the spin column, and centrifuge at 700 x g (approx. 2’000rpm) for 2min.
2. Discard the eluate, load up to 0.5ml of cell lysate, centrifuge for 2min, and collect the flow-through fraction.
3. Add 0.5ml of washing buffer, centrifuge for 2min, and collect the wash fraction.
4. Repeat step 3, if necessary.
5. Add 0.2ml of elution buffer and centrifuge.
6. Collect the eluate. |
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FIGURES
Figure: Principle of SpinClean™ MBP Excellose ® Kit (Prod. No. MBI-23020 (20 preparations) and MBI-23050 (50 preparations)).
Figure: MBP fusion protein pruification
Method: E. coli lysate was loaded to MBP Excellose® spin column.
Lane 1: E. coli lysate
Lane 2: Flow-through fraction
Lane 3: Wash fraction
Lane 4: Purified MBP
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United States
