5(6)-Carboxyeosin (CE) is a brominated analog of carboxyfluorescein that is a good photosensitizer and singlet oxygen generator. Carboxyeosin has a singlet oxygen yield that is approximately 19 times greater than that of fluorescein. Using this property, CE has been found to be useful for photoconverting diaminobenzidine (DAB) as a method for improving resolution for immunoprecipitation or in situ hybridization techniques in light and electron microscopy. Upon excitation, carboxyeosin labeled probes can be used to generate singlet oxygen, which in turn will oxidize DAB into an opaque precipitate within cells.
|Solubility:||Soluble in DMF, DMSO or water (at pH>6).||