Direct fluorescence-based assays for detecting metallo-, serine, acid or sulfhydryl proteases are important in medical, biochemical and cell biology research. Analysis of low levels of protease activity is important in biochemical quality control testing, for analysis of protease inhibitors or cofactors, as well as for basic research application in biology and molecular biology. Several fluorescence-based methods have been developed for detecting protease activity including the fluorescein thiocarbamoyl (FTC)-casein protease assay, in which unhydrolyzed protein must be precipitated with trichloroacetic acid, separated by centrifugation, transferred for measurement and then pH-adjusted to optimize the fluorescence signal.
|Application Notes:||Allows quick and simple measurement of general protease activity in biological samples.|
|Background / Technical Information:||For the Manual please click here.||