|Background / Technical Information:|
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Note: This assay was developed and optimised for purified TG2. Using undefined probes, e.g. cell homogenate, the assay can be seriously influenced. This is probably due to e.g. proteases which cleave the peptidic backbone of the substrate, resulting in increased fluorescence independently of TG2 activity. Therefore we recommend to perform suitable controls e.g. by inhibition of TG2 using suitable inhibitors.