Catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. It may also be used for immunoprecipitation.
|Alternative Name:||TG1, TGase 1, Keratinocyte protein-glutamine-γ-glutamyltransferase|
|Sequence:||Based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to His6-tag.|
|Source:||Produced in insect cells.|
|Formulation:||Lyophilized from 50mM Tris-HCl, pH 8.0, containing 10mM Glutathione.|
|Purity Detail:||Purified by Ni2+-affinity chromatography.|
|Activity:||Add 10mM Ca2+ to activate TG1.|
> 8,000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG1-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex =332nm, λem =500nm; band filter =5nm; detector strength =600V; temperature =37°C, assay volume =1ml)].
|Reconstitution:||Reconstitute with water. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage.|
|Long Term Storage:||-20°C|
|Handling:||After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.|
|Background / Technical Information:||For the Original Manufacturer’s Data Sheet please click here. |
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.