Transglutaminases catalyze acyl transfer reactions from glutamine residues in proteins or peptides to primary amines. The transglutaminase-catayzed covalent coupling of monodansylcadaverine into N,N-dimethylcasein produces a shift in intensity and wavelength of fluorescence of the dansyl group. The transglutaminase activity can be monitored online by measurement of the fluorescence (excitation wavelength 332 nm; emission wavelength 500 nm). The relative transglutaminase activity is shown by increase of fluorescence intensity over time.
|Application Notes:||For the determination of human transglutaminase activity (hTG1, hTG2 and hTG6).|
|Use/Stability:||The unopened reagents are stable until the expiration date printed on the box when stored at +4°C.|
|Handling:||Protect from light.|
|Long Term Storage:||+4°C|
|Kit/Set Contains:||Substrate reagent: 2x 0.185mg monodansylcadaverine|
Buffer reagent: 2x 20ml lyophilized TRIS, pH 8.0, containing calcium chloride, sodium chloride, N,N-dimethylcasein and glutathione.
|Background / Technical Information:||For the Manual please click here.||