Recombinant rat Factor XIII is a homodimer (a2) composed of two chains held together by non covalent bonds. After activation of the zymogen by Thrombin and Ca2+ to its active form (a*2, Factor XIIIa), Factor XIIIa catalyzes the formation of covalent bridges (ε-(γ-glutamyl)lysine bonds) between fibrin units to increase the elasticity of the clot network. The resulting crosslinked fibrin is insoluble and resistant to lysis.
|Alternative Name:||Fibrin Stabilizing Factor|
|MW:||~83kDa (monomer); ~166kDa (homodimer)|
|Source:||Produced in insect cells.|
|Formulation:||Lyophilized from 20mM TRIS-HCl, pH 7.5, 150mM sodium chloride, 1mM EDTA and 1mM DTT. Sample contains maltodextrin.|
|Application Notes:||Catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e.g. the formation of ε-(γ-glutamyl)lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.|
|Reconstitution:||Add the desired volume of water (e.g. 400 μL) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage. Storage for several weeks is possible at ≤ - 20°C.|
|Background / Technical Information:||For the Original Manufacturer’s Data Sheet please click here. |
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.