Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand (CD95L) of the TNF family through their death domain containing receptors, TNF-R1 and Fas (CD95). A novel member in the TNF family was identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand) [1,2]. TRAIL is a type II membrane protein and expressed in a variety of human tissues.
Two novel death domain containing receptors TRAIL-R1 (DR4) and TRAIL-R2 (DR5) have been identified as the receptor for TRAIL [3-6]. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-κB activation in many tissues and cells. TRAIL also binds to TRAIL-R3 (DcR1) and TRAIL-R4 (DcR2) [7,8].
PRODUCT PROPERTIES |
Alternative Name: | | Apo-2L, TNFSF 10, CD253 |
Host: | | Rabbit |
Immunogen: | | Synthetic peptide (PSC-1113P) corresponding to C-terminal (aa 261-277 human TRAIL. |
Species reactivity: | | Human
|
Applications: | | WB
|
Application Notes: | | Detects a band of ~32kDa by Western blot. |
Positive Control: | | HeLa whole cell lysate (Prod. No. PSC-1201) can be used as positive control. |
Blocking Peptide: | | For Blocking Peptide see Prod. No. PSC-1113P.
|
Formulation: | | Liquid. 100µg purified rabbit IgG in 200µl PBS containing 0.02% sodium azide. |
Long Term Storage: | | +4°C |
Background / Technical Information: | | For the Original Manufacturer's data sheet please click here. |
|
|
FIGURES
Figure: Western blot analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with anti-TRAIL (CT) at 1µg/ml.
|
Product Literature References
A novel receptor for Apo2L/TRAIL contains a truncated death domains: S.A. Marsters, et al.; Curr. Biol.
7, 1003 (1997),
Abstract;
An antagonist decoy receptor and a death domain-containing receptor for TRAIL: G. Pan et al.; Science
277, 815 (1997),
Abstract;
Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors: J.P. Sheridan et al.; Science
277, 818 (1997),
Abstract;
The novel receptor TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated apoptosis, yet retains an incomplete death domain: M.A. Degli-Esposti, et al.; Immunity
7, 813 (1997),
Abstract;
The receptor for the cytotoxic ligand TRAIL: G. Pan et al.; Science
276, 111 (1997),
Abstract;
TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-kB: P. Schneider et al.; Immunity
7, 831 (1997),
Abstract;
Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family: R.M. Pitti et al.; J. Biol. Chem.
271, 12687 (1996),
Abstract;
Full Text
Identification and characterization of a new member of the TNF family that induces apoptosis: S.R. Wiley et al.; Immunity
3, 673 (1995),
Abstract;