Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand (CD95L) of the TNF family through their death domain containing receptors, TNF-R1 and Fas (CD95). A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4) [1]. The ligand for this novel death receptor has been identified and termed TRAIL [2,3], which is a member in the TNF family.
DR4 is also called TRAIL receptor-1 (TRAIL-R1) [4]. DR4 is expressed in most of human tissues including spleen, small intestine, thymus and peripheral blood leukocytes. Like TNF-R1, Fas and DR3, TRAIL-R1 mediates apoptosis and NF-κB activation in many tissues and cells.
PRODUCT PROPERTIES |
Alternative Name: | | DR4, TNFRSF 10A |
Immunogen: | | Synthetic peptide (PSC-1167P) corresponding to N-terminal (aa 1-20) human TRAIL-R1 mature protein. |
Source: | | From rabbit. |
Species reactivity: | | Human
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Applications: | | WB
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Application Notes: | | Detects a band of ~57kDa by Western blot. |
Positive Control: | | HeLa whole cell lysate (Prod. No. PSC-1201) or Jurkat whole cell lysate (Prod. No. PSC-1205) can be used as positive control. |
Blocking Peptide: | | For Blocking Peptide see Prod. No. PSC-1167P.
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Formulation: | | Liquid. 100µg purified rabbit IgG in 200µl PBS containing 0.02% sodium azide. |
Long Term Storage: | | +4°C |
Background / Technical Information: | | For the Original Manufacturer's data sheet please click here. |
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FIGURES
Figure: Western blot analysis of DR4 in HeLa (H), K562 (K), and Jurkat (J) whole cell lysate with anti-DR4 (NT) at 1:500 dilution.
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General Literature References
The receptor for the cytotoxic ligand TRAIL : G. Pan, et al.; Science
276, 111 (1997),
Abstract;
TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-kappaB : P. Schneider, et al.; Immunity
7, 831 (1997),
Abstract;
Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family : R.M. Pitti, et al.; J. Biol. Chem.
271, 12687 (1996),
Abstract;
Full Text
Identification and characterization of a new member of the TNF family that induces apoptosis : S.R. Wiley, et al.; Immunity
3, 673 (1995),
Abstract;