Rat Tissue Blot I is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 µg of total cellular protein in each lane. Proteins in a wide range of molecular mass are separated in a 4-20% gradient SDS-PAGE gel and transferred onto nitrocellulose membranes.
Custom gel percentages between 7% and 15% may be available upon request.
The rat tissues included in Rat Tissue Blot I are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.
PRODUCT PROPERTIES |
Source: | | The Outbred Sprague Dawley Albino rat tissues included in the blot are: brain, colon, heart, kidney, liver, lung, pancreas, and spleen. |
Applications: | | WB
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Application Notes: | | For western blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature. |
Long Term Storage: | | +4°C |
Background / Technical Information: | | For the Original Manufacturer's data sheet please click here. |
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FIGURES
Figure: Coomassie blue staining of rat tissue lysates in 10% SDS-PAGE. Lanes: 1, protein markers; 2, brain; 3, colon; 4, heart; 5, kidney; 6, liver; 7, lung; 8, pancreas; and 9, spleen.
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