Apoptosis is related to many diseases and can also be induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and caspase-10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively [1,2]. The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and Usurpin, respectively [3-8].
FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter proteins FADD and caspase-8 and caspase-10, and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R and TNF-R1. Four splice variants of c-FLIPs have been identified and termed FLIPα, β, γ, and δ, respectively [9].
PRODUCT PROPERTIES |
Immunogen: | | Synthetic peptide (Prod.No. PSC-2055P) corresponding to C-terminal (aa 191-209) human FLIPδ/FLIPs form [2]. |
Source: | | From rabbit. |
Species reactivity: | | Human, Mouse
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Specificity: | | Recognizes FLIPγ and FLIPδ in human and mouse. |
Crossreactivity: | | Does not cross-react with FLIPα or FLIPβ. |
Applications: | | WB
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Application Notes: | | Detects a band of ~25kDa and ~35kDa by Western blot. |
Positive Control: | | Jurkat whole cell lysate (Prod. No. PSC-1205) can be used as positive control. |
Blocking Peptide: | | For Blocking Peptide see Prod. No. PSC-2055P.
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Formulation: | | Liquid. 100µg purified IgG in 200µl PBS containing 0.02% sodium azide. |
Long Term Storage: | | +4°C |
Background / Technical Information: | | For the Original Manufacturer's data sheet please click here. |
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FIGURES
Figure: Western blot analysis of FLIPg/d in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with anti-FLIPg/d (CT) at 1:1000 dilution.
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Product Literature References
Cell death attenuation by 'Usurpin', a mammalian DED-caspase homologue that precludes caspase-8 recruitment and activation by the CD-95 (Fas, APO-1) receptor complex: D.M. Rasper, et al.; Cell Death Differ.
5, 271 (1998),
Abstract;
Inhibition of Fas death signals by FLIPs: J. Tschopp, et al.; Curr. Opin. Immunol.
10, 552 (1998),
Abstract;
CASH, a novel caspase homologue with death effector domains: Y.V. Goltsev, et al.; J. Biol. Chem.
272, 19641 (1997),
Abstract;
Full Text
Casper is a FADD- and caspase-related inducer of apoptosis: H.B. Shu, et al.; Immunity
6, 751 (1997),
Abstract;
CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis: N. Inohara, et al.; PNAS
94, 10717 (1997),
Abstract;
Full Text
FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis: S.M. Srinivasula, et al.; J. Biol. Chem.
272, 18542 (1997),
Abstract;
Full Text
I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1 and CD-95-induced apoptosis: S. Hu, et al.; J. Biol. Chem.
272, 17255 (1997),
Abstract;
Full Text
Inhibition of death receptor signals by cellular FLIP: M. Irmler, et al.; Nature
388, 190 (1997),
Abstract;
Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors: M. Thome, et al.; Nature
386, 517 (1997),
Abstract;