Nuclear factor kappa B (NF-κB) is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories [1-5]. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα [IKK1] and IKKβ [IKK2] interact with each other and both are essential for the NF-κB activation.
IKKα specifically phosphorylates IκB-α. IKKα is expressed in a variety of human tissues.
PRODUCT PROPERTIES |
Alternative Name: | | Inhibitor of Nuclear Factor κ-B Kinase α, IKK1 |
Host: | | Rabbit |
Immunogen: | | Synthetic peptide corresponding to aa 658-674 (C658TQSSARSLVGSSLEGA674) of human IKKα (inhibitor of NF-κB kinase α; IKK1). |
Species reactivity: | | Human
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Crossreactivity: | | Does not cross-react with IKKβ or IKKγ. |
Applications: | | WB
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Application Notes: | | Detects a band of ~85kDa by Western blot. |
Positive Control: | | Jurkat whole cell lysate (Prod. No. PSC-1205) can be used as positive control. |
Blocking Peptide: | | For Blocking Peptide see Prod. No. PSC-2117P.
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Formulation: | | Liquid. 100µg purified rabbit IgG in 200µl PBS containing 0.02% sodium azide. |
Long Term Storage: | | +4°C |
Background / Technical Information: | | For the Original Manufacturer's data sheet please click here. |
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FIGURES
Figure: Western blot analysis of IKKa in HeLa whole cell lysate with anti-IKKa (C2) at 1:500 dilution.
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Product Literature References
A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB: J.A. DiDonato, et al.; Nature
388, 548 (1997),
Abstract;
Identification and characterization of an IkappaB kinase: C.H. Regnier, et al.; Cell
90, 373 (1997),
Abstract;
IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK: J.D. Woronicz, et al.; Science
278, 866 (1997),
Abstract;
IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation: F. Mercurio, et al.; Science
278, 860 (1997),
Abstract;
The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation: E. Zandi, et al.; Cell
91, 243 (1997),
Abstract;