Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aα and FEM1β [1,2]. F1Aα/FEM1β is the homologue of C. elegans sex determining protein FEM-1 [1,2]. FEM-1/F1Aα is cleaved by CED-3 and caspase . FEM-1/F1Aα associates with CED-4 and its mammalian homologue Apaf-1 . Overexpression of F1Aα induces apoptosis.
|Immunogen:||Synthetic peptide (Prod.No. PSC-2279P) corresponding to aa 609-622 of human F1Aα . This sequence is identical to the corresponding sequence of mouse FEM1β .|
|Species reactivity:||Human, Mouse, Rat|
|Application Notes:||Detects a band of ~70kDa by Western blot.|
|Positive Control:||Murine liver tissue lysate (Prod.No. PSC-1404) can be used as positive control.|
|Blocking Peptide:||For Blocking Peptide see Prod. No. PSC-2279P.|
|Formulation:||Liquid. 100µg purified IgG in 200µl PBS containing 0.02% sodium azide.|
|Long Term Storage:||+4°C|
|Background / Technical Information:||For the Original Manufacturer's Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer's Data Sheet.
Figure: Western blot analysis of F1Aa in mouse (A) and rat (B) liver tissue lysates with anti- F1Aa at 1µg /ml.
Product Literature References
Sequence, organization, and expression of the human FEM1B gene
: T. Ventura-Holman and J.F. Maher; BBRC 267
, 317 (2000), Abstract
The Caenorhabditis elegans sex determination protein FEM-1 is a CED-3 substrate that associates with CED-4 and mediates apoptosis in mammalian cells
: S.L. Chan, et al.; J. Biol. Chem. 275
, 17925 (2000), Abstract
; Full Text
F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis
: S.L. Chan, et al.; J. Biol. Chem. 274
, 32461 (1999), Abstract
; Full Text