Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB associates with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-κB activator 1) [1,2].
|Alternative Name:||anti-NF-κB Activator (human) (CT) PAb, anti-Connection to IKK and SAPK/JNK (human) (CT) PAb|
|Immunogen:||Synthetic peptide corresponding to C-terminal aa 554-568 (R554EEEYVAPPRGPLPT568) of human Act1/CIKS [1,2].|
|Application Notes:||Detects a band of ~63kDa by Western blot. |
|Positive Control:||Human placenta tissue lysate can be used as positive control. |
|Blocking Peptide:||For Blocking Peptide see Prod. No. PSC-2499P.|
|Formulation:||Liquid. 100µg immunoaffinity chromatography purified IgG in 200 µl PBS containing 0.02% sodium azide.|
|Long Term Storage:||+4°C|
|Background / Technical Information:||For the Original Manufacturer's Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer's Data Sheet.
Figure: Western blot analysis of CIKS expression in human placenta tissue lysate with anti-CIKS (CT) at 1µg /ml.
Product Literature References
Act1, an NF-kappa B-activating protein
: X. Li, et al.; PNAS 97
, 10489 (2000), Abstract
; Full Text
CIKS, a connection to Ikappa B kinase and stress-activated protein kinase
: A. Leonardi, et al.; PNAS 97
, 10494 (2000), Abstract
; Full Text