Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and 7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis .
|Alternative Name:||Anti-PHAP (NT) PAb, Anti-HLA-DR associated Protein (NT) PAb|
|Immunogen:||Synthetic peptide corresponding to the N-terminus of human PHAP .|
|Species reactivity:||Human, Mouse, Rat|
|Specificity:||Recognizes human, mouse and rat PHAP I, PHAP I2a, and PHAP III.|
|Positive Control:||Raji cell lysate (Prod. No. PSC-1207) can be used as positive control.|
|Blocking Peptide:||For Blocking Peptide see Prod. No. PSC-3149P.|
|Formulation:||Liquid. 100µg purified IgG in 100µl PBS containing 0.02% sodium azide.|
|Use/Stability:||Stable for one year when stored at 4°C.|
|Long Term Storage:||+4°C|
|Background / Technical Information:||For the Original Manufacturer's Data Sheet please click here.|
Our product description may differ slightly from the Original Manufacturer's Data Sheet.
Figure: Western blot analysis of PHAP expression in human Raji cell lysate with anti-PHAP PAb at 1 µg/ml. The wide and strong band below PHAP III is a doublelet composed of PHAP I (upper) and PHAP I2a (lower).
Product Literature References
Apoptosis. Life and death decisions
: D.W. Nicholson and N.A. Thornberry; Science 299
, 214 (2003), Abstract
Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway
: X. Jiang, et al.; Science 299
, 223 (2003), Abstract