This mutant is lacking binding to VEGFR-2 (KDR) and can serve as a unique tool to investigate the signal transduction of VEGF-C via VEGFR-3 (Flt-4). According to the high degree of homology between growth factors of mammalian species, the protein can also be used for experiments with human cells and for further in vitro and in vivo experiments with other cells and species.
PRODUCT PROPERTIES |
Alternative Name: | | VEGF-C (C152S mutant) (rat) (recombinant) (His) |
MW: | | ~18-24kDa. |
Source: | | Produced in insect cells. |
Formulation: | | Lyophilized. |
Purity: | | ≥90% (SDS-PAGE) |
Endotoxin Content: | | ≤1.2EU/µg protein. |
Specific Activity: | | ~5x103 U/mg. |
Biological Activity: | | Stimulates phosphorylation of VEGFR-3 in pig aortic endothelial cells (ED50=150-300ng/ml). |
Solubility: | | Soluble in water and most aqueous buffers. |
Reconstitution: | | Reconstitute with PBS or medium (>50µg/ml). |
Short Term Storage: | | +4°C |
Long Term Storage: | | -20°C |
Handling: | | After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
Background / Technical Information: | | For the Original Manufacturer's data sheet please click here. |
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Product Literature References
Differential in vivo and in vitro expression of vascular endothelial growth factor (VEGF)-C and VEGF-D in tumors and its relationship to lymphatic metastasis in immunocompetent rats: J. Krishnan, et al.; Cancer Res.
63, 713 (2003),
Abstract;
Lymphangiogenic gene therapy with minimal blood vascular side effects: A. Saaristo, et al.; J. Exp. Med.
196, 719 (2002),
Abstract;
Characterization of indolinones which preferentially inhibit VEGF-C- and VEGF-D-induced activation of VEGFR-3 rather than VEGFR-2: V. Kirkin, et al.; Eur. J. Biochem.
268, 5530 (2001),
Abstract;
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Signalling via vascular endothelial growth factor receptor-3 is sufficient for lymphangiogenesis in transgenic mice: T. Veikkola, et al.; EMBO J.
20, 1223 (2001),
Abstract;
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Vascular endothelial growth factor-C stimulates the migration and proliferation of Kaposi's sarcoma cells: S. Marchio, et al.; J. Biol. Chem.
274, 27617 (1999),
Abstract;
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A recombinant mutant vascular endothelial growth factor-C that has lost vascular endothelial growth factor receptor-2 binding, activation, and vascular permeability activities: V. Joukov, et al.; J. Biol. Chem.
273, 6599 (1998),
Abstract;
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