Cathepsin K Activity Assay Kit |
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| PRODUCT LINE | Cancer |
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| PRODUCT FAMILIES | Cathepsin Kits & Sets |
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| Ordering Information |
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| Product Numbers | Format | Size | Unit Price | Quantity | | | BV-K141-100 |
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100 Assays | | | | | | |
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| Product Specification |
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| APPLICATION: | For the quantification of cathepsin K activity by fluorometry. |
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| SHIPPING: | SHIPPED ON BLUE ICE |
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| LONG TERM STORAGE: | -20°C |
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| HANDLING: | Protect from light. |
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| Product Description |
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin K Activity Assay Kit is a fluorescence-based assay that utilizes the preferred cathepsin K substrate sequence LR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The cathepsin K assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity. |
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| | General Information | |
Background/Technical Information | | For the Original Manufacturer’s Data Sheet please click here.
Our product description may differ slightly from the Original Manufacturer’s Data Sheet. | | |
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| RELATED PRODUCT GROUPS |
Kits & Sets > Kits > Activity Assays / Bone Metabolism > Bone Metabolism Other Products |
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