CD40L, soluble (human) (recombinant) set |
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| SYNONYMS | CD154, soluble (human) (recombinant) set
TNFSF 5, soluble (human) (recombinant) set |
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| PRODUCT LINE | Chemokines & Cytokines |
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| PRODUCT FAMILIES | CD40 & CD40L [CD154] / Related Products |
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| Ordering Information |
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| Product Numbers | Format | Size | Unit Price | Quantity | | | ALX-850-064-KI01 |
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1 Set | | | | | | |
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| Product Specification |
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| SPECIES CROSSREACTIVITY: | |
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| SOURCE/HOST: | Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag. |
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| CONCENTRATION: | CD40L: 0.1mg/ml after reconstitution.
Enhancer: 1mg/ml after reconstitution. |
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| KIT/SET CONTAINS: | 10µg of CD40L, Soluble (human) (recombinant) (Prod. No. ALX-522-015) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034), which increases the biological activity of rhsCD40L at least 1’000-fold. |
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| PURITY: | ≥95% (SDS-PAGE). |
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| FORMULATION: | Lyophilized. Contains PBS. |
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| RECONSTITUTION: | Reconstitute CD40L with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein. |
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| SPECIFICITY: | CD40L binds to human CD40. |
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| APPLICATION: | Can be used to activate human B cells and dendritic cells. Stimulates growth of human B cells. For stimulation of mouse cells via CD40 use MAb to CD40 (mouse) (FGK45) (Prod. No. ALX-805-046). |
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| SHIPPING: | SHIPPED ON BLUE ICE |
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| LONG TERM STORAGE: | -20°C |
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| USE/STABILITY: | Stable for at least 6 months after receipt when stored at -20°C. |
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| HANDLING: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| | Product Specific Literature References | Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Full Text; http://www.jem.org/cgi/content/full/187/8/1205
CD45 inhibits CD40L-induced microglial activation via negative regulation of the Src/p44/42 MAPK pathway: J. Tan, et al.; J. Biol. Chem. 275, 37224 (2000) Full Text; http://www.jbc.org/cgi/content/full/275/47/37224
CD40 induces apoptosis in carcinoma cells through activation of cytotoxic ligands of the tumor necrosis factor superfamily: A.G. Eliopoulos, et al.; Mol. Cell. Biol. 20, 5503 (2000) Abstract
Brain dendritic cells and macrophages/microglia in central nervous system inflammation: H.G. Fischer & G. Reichmann; J. Immunol. 166, 2717 (2001) Full Text; http://www.jimmunol.org/cgi/content/full/166/4/2717
Synergy between CD40 ligation and IL-4 on fibroblast proliferation involves IL-4 receptor signaling: S.P. Atamas, et al.; J. Immunol. 168, 1139 (2002) Full Text; http://www.jimmunol.org/cgi/content/full/168/3/1139
The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines: A. Gjorloff Wingren, et al.; J. Immunol. 168, 5582 (2002) Full Text; http://www.jimmunol.org/cgi/content/full/168/11/5582
Platelet-Activating Factor Mediates CD40-Dependent Angiogenesis and Endothelial-Smooth Muscle Cell Interaction: S. Russo, et al.; J. Immunol. 171, 5489 (2003) Abstract
Induction of antigen-specific CTL by recombinant HIV trans-activating fusion protein-pulsed human monocyte-derived dendritic cells: Y. Tanaka, et al.; J. Immunol. 170, 1291 (2003) Full Text; http://www.jimmunol.org/cgi/content/full/170/3/1291
IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo: A. Soruri, et al.; J. Immunol. 170, 3306 (2003) Full Text; http://www.jimmunol.org/cgi/content/full/170/6/3306
CD40 induces human multiple myeloma cell migration via phosphatidylinositol 3-kinase/AKT/NF-kappa B signaling: Y.T. Tai, et al.; Blood 101, 2762 (2003) Abstract
TNF receptor-associated factor 6 is an essential mediator of CD40-activated proinflammatory pathways in monocytes and macrophages: L. Mukundan, et al.; J. Immunol. 174, 1018 (2005) Full Text; http://www.jimmunol.org/cgi/content/full/174/2/1081
Enhanced activation of human dendritic cells by inducible CD40 and Toll-like receptor-4 ligation: N. Lapteva, et al.; Cancer Res. 67, 10528 (2007) Abstract |
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| RELATED PRODUCT GROUPS |
Kits & Sets > Sets > Receptor / Ligand + Enhancer Set / Immunology > CD Markers > CD Kits & Sets |
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