SpinClean™ Genomic DNA Purification Kit (for bacteria samples) |
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| PRODUCT LINE | Molecular Biology |
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| PRODUCT FAMILIES | DNA/RNA Extraction & Purification |
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| Ordering Information |
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| Product Numbers | Format | Size | Unit Price | Quantity | | | MBI-16200 |
200 preparations |
1 Kit | | | | | | |
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| Product Specification |
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| KIT/SET CONTAINS: | SpinClean™ Column: 200ea
Collection Tube: 200ea
GLB(gDNA Lysis Buffer): 40ml
Binding Buffer: 60ml
Wash Solution I: 50ml
Wash Solution ll: 24ml
Elution Buffer: 20ml
Proteinase K: 4ml
RNase A: 800µl |
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| Product Description |
The SpinClean™ Genomic DNA purification kit are designed for rapid isolation of genomic DNA from various sample sources including whole blood cells (for blood sample), Gram positive and Gram negative bacteria (for bacteria sample). This system is a fast, simple and inexpensive method. The SpinClean™ Genomic DNA purification kit use advanced silica-based membrane technology for rapid and efficient purification of genomic DNA without organic extraction or ethanol precipitation. DNA purified with this system is suitable for a variety of application, including amplifications, membrane hybridizations, and digestion with restriction endonuclease. |
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| | General Information | |
Background/Technical Information | | Recommended storage condition: Store at room temperature (Proteinase K is stable at 4°C and recommend storage at -20°C. Binding buffer is stored at 4°C) .
PROTOCOL
Gram Negative Bateria
1. Pellet 1.5ml of bacterial culture by centrufugation (8000rpm for 5min).
2. Add 200µl of Lysis buffer and 20ul of Proteinase K and then mix well by vortexing.
3. Incubate for 15min at 65°C.
[optional] RNase treatment : Add 4ul of RNase (100mg/ml) and wait for 2 min at RT.
4. Add 300µl of Binding buffer and mix well by vortexing or pipeting.
5. Place a SpinClean™ Column in a 2ml Collection Tube.
6. Transfer the sample to the SpinClean™ Column.
7. Centrifuge for 1min at 13000rpm. Discard the flow-through.
8. Wash the SpinClean™ Column by adding 500µl of Wash Solution Ⅰ*.
9. Centrifuge for 1min at 13000rpm.
10. Add 600µl of Wash Solution Ⅱ* and centrifuge for 2min at maximum speed.
11. Place SpinClean™ Column in a clean 1.5 ml microfuge tube.
12. To elute DNA, add 100µl of prewarmed Elution buffer or H2O on the center of SpinClean™ Column, wait for 1min, and then centrifuge for 1 min.
Gram Positive Bateria
1. Pellet 1.5ml of bacterial culture by centrufugation (8000rpm for 5min).
2. Add 200µl of Lysozyme buffer** and incubate for (≥30min) at 37°C.
[optional] RNase treatment : Add 4µl of RNase (100mg/ml) at this step.
3. Add 300µl of Binding buffer and 20µl Proteinase K and then mix by vortexing.
4. Incubate for 15min at 70°C and cooling.
5. Continue step 5 of gram negative bacteria.
CAUTION
- Proteinase K is stable at 4°C and recommend storage at -20°C.
- Binding buffer is stored at 4°C.
* Add 95% ethanol in Wash Solution (Ⅰ, Ⅱ ) before use.
** Lysozyme buffer (20mM Tris-Cl (pH 8.0), 2mM EDTA, 1.2% Triton X-100, 20mg/ml lysozyme)
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| RELATED PRODUCT GROUPS |
Kits & Sets > Kits > Molecular Biology Kits |
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