Ac-DEVD-AFC |
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| SYNONYMS | Caspase-3 Substrate (Fluorogenic) |
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| PRODUCT LINE | Cell Death / Apoptosis / Autophagy |
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| PRODUCT FAMILIES | Caspase Substrates |
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| Ordering Information |
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| Product Numbers | Format | Size | Unit Price | Quantity | | | ALX-260-032-M001 |
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1 mg | | | | | ALX-260-032-M005 |
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5 mg | | | | | | |
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| Product Specification |
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| SEQUENCE: | Ac-Asp-Glu-Val-Asp-AFC (AFC = 7-Amino-4-trifluoromethylcoumarin) |
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| FORMULA: | C30H34F3N5O13 |
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| MW: | 729.6 |
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| PEPTIDE CONTENT: | 65-95% |
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| PURITY: | ≥96% (HPLC) |
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| APPEARANCE: | White to off-white powder. |
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| SOLUBILITY: | Soluble in dimethyl formamide, DMSO or methanol; slightly soluble in water (0.4mg/ml). |
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| SHIPPING: | AMBIENT |
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| LONG TERM STORAGE: | -20°C |
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| HANDLING: | Keep cool and dry. |
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| Product Description |
Fluorogenic substrate for caspase-3. Also a substrate for caspase-1, -4 and -7. This product is similar to Ac-DEVD-AMC (Prod. No. ALX-260-031) but the AFC fluorophore has a greater Stokes’ shift upon cleavage. The peptide sequence is based on the PARP cleavage site Asp216 for caspase-3. |
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| | Product Specific Literature References | BAX-induced cell death may not require interleukin 1 β-converting enzyme-like proteases: J. Xiang, et al.; Proc. Natl. Acad. Sci. USA 93, 14559 (1996) Abstract
Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver: J.M. Chandler, et al.; J. Biol. Chem. 273, 10815 (1998) Full Text; http://www.jbc.org/cgi/reprint/273/18/10815.pdf
Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP: M. Marcelli, et al.; Cancer Res. 58, 76 (1998) Abstract |
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| | General Information | |
Background/Technical Information | | AFC has an excitation maximum of 400nm and an emission maximum of 505nm. | | |
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| RELATED PRODUCT GROUPS |
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