Carbohydrate Analysis/Detection Kit |  | | PRODUCT LINE | Protein Expression / Modification | | | | PRODUCT FAMILIES | Protein Glycosylation & Glycoproteins Other Products | | | | | Ordering Information | | | Product Numbers | Format | Size | Unit Price | Quantity | | | MGT-M0272-KI01 |
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1 Kit | | | | | | |
| | | Product Specification | | |
| KIT/SET CONTAINS: | Sample preparation:
Fluorescent reagent: 1 x 2.0ml vial
(20mM 1,5-EDANS in 0.1N NaHCO3 at pH 10 with NaOH).
Reduction reagent: 1 x 76mg vial
(Na-Borohydride)
Reduction reagent solvent: 1 x 2.0 ml vial
(DMSO to dissolve Na-Borohydride to a 1.0M solution).
TLC analysis:
TLC solvent A: 25ml
TLC solvent B: 25ml
TLC plates: 3 | | | | | | Product Description | Numerous native proteins contain post-transcriptional glycosidic elaboration (sugars) whose structures are dependent both on species and cell type. The Carbohydrate Analysis/Detection Kit is capable of quickly estimating and/or comparing the composition of the carbohydrates from these glycoproteins. The Kit provides reagents and protocols for analyzing these carbohydrates through covalent labeling with a fluorescent reagent (1,5-EDANS). |
| | | | | General Information | |
Background/Technical Information | | Numerous native proteins contain post-transcriptional glycosidic elaboration (sugars) whose structures are dependent both on species and cell type. The characterization of the complex oligosaccharides obtained from these glycoproteins has proven a difficult and time consuming endeavor [1,2]. The Carbohydrate Analysis/Detection Kit is capable of quickly estimating and/or comparing the composition of the carbohydrates in such samples. The Kit provides reagents and protocols for analyzing these carbohydrates through covalent labeling with a fluorescent reagent (1,5-EDANS), and analysis by thin-layer chromatography (TLC). The principle involves enzymatic removal of the oligosaccharides from a native protein (or mixture of reducing sugars), reductive amination of the reducing sugars and analysis of the resultant glycamines using silica-gel two dimensional thin layer chromatography (2D-TLC) or by other well established techniques [1,2]. The advantages of using the 1,5 EDANS fluorophore include its low detection limit, water solubility, pH fluorescence invariance, stability, distinctive fluorescence from protein chromophores, and ability to be detected using normal phase chromatography techniques.
We anticipate the use of this new kit to also aid other carbohydrate analyses including HPLC detection and purification, carbohydrate receptor analyses, enzyme inhibition studies, and the like.
For the Original Manufacturer’s Data Sheet please click here.
Our product description may differ slightly from the Original Manufacturer’s Data Sheet. | | | | GENERAL LITERATURE REFERENCES |
[1] The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid. Detection of picomolar quantities by an imaging system ba: P. Jackson; Biochem. J. 270, 705 (1990) Abstract;
[2] Analysis of glycopeptides as borate complexes by polyacrylamide gel electrophoresis: S. Weitzman, et al.; Anal. Biochem. 97, 438 (1979) Abstract;
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