Mitochondria/Cytosol fractionation kit |
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| PRODUCT LINE | Cell Death / Apoptosis / Autophagy |
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| PRODUCT FAMILIES | Mitochondrial Pathway Other Products |
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| Ordering Information |
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| Product Numbers | Format | Size | Unit Price | Quantity | | | ALX-850-276-KI01 |
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1 Kit | | | | | | |
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| Product Specification |
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| QUANTITY: | ~100 tests. |
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| KIT/SET CONTAINS: | 10ml Mitochondria Extraction Buffer, 20ml 5X Cytosol Extraction Buffer, 110µl DTT (1M), 1 vial Protease Inhibitor Cocktail. |
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| APPLICATION: | For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays. |
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| SHIPPING: | SHIPPED ON BLUE ICE |
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| LONG TERM STORAGE: | -20°C |
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| HANDLING: | Protect from light. Avoid freeze/thaw cycles. |
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| HAZARD: | HARMFUL. |
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| Product Description |
The core kit contains unique formulations of buffers and reagents that allow isolation of mitochondria and cytosol in only two easy steps. No ultracentrifugations are required. No toxic chemicals are involved. |
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| | Product Specific Literature References | | Green tea epigallocatechin 3-gallate accumulates in mitochondria and displays a selective antiapoptotic effect against inducers of mitochondrial oxidative stress in neurons: E.K. Schroeder, et al.; Antioxid. Redox Signal 11, 469 (2009) Abstract |
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| RELATED PRODUCT GROUPS |
Kits & Sets > Kits > Kits Other Products |
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