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 ALX-522-059[ALEXIS] Revised 02-Mar-07  

Adiponectin (mouse) (recombinant)

SYNONYMS   

ACRP30 (mouse) (recombinant)
Adipocyte Complement Related Protein of 30kDa (mouse) (recombinant)
AdipoQ (mouse) (recombinant)
apM1 (mouse) (recombinant)
GBP28 (mouse) (recombinant)

PRODUCT LINE   

Obesity & Adipokines

PRODUCT FAMILIES   

Adiponectin [ACRP30] / Related Products

Ordering Information
 Product NumbersFormatSizeUnit PriceQuantity
 ALX-522-059-C050 50 µg  
Product Specification
SPECIES CROSSREACTIVITY:   
Mouse
 
MW:   ~35kDa (SDS-PAGE).
 
SOURCE/HOST:   Produced in HEK 293 cells. Mouse adiponectin (aa 18-247) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
 
CONCENTRATION:   0.5mg/ml after reconstitution.
 
PURITY:   ≥90% (SDS-PAGE)
 
FORMULATION:   Lyophilized. Contains PBS.
 
ENDOTOXIN CONTENT:   <0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
RECONSTITUTION:   Reconstitute with 100µl sterile water.
 
BIOLOGICAL ACTIVITY:   Inhibits serum glucose production in mice. Protects mouse hepatocytes from TNF-α-induced cell death in vitro.
 
SHIPPING:   SHIPPED ON BLUE ICE
 
LONG TERM STORAGE:   -20°C
 
USE/STABILITY:   Stable for at least 6 months after receipt when stored at -20°C.
 
HANDLING:   Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Description

Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species.

Product Specific Literature References
Regulation of T-cell mediated hepatic inflammation by adiponectin and leptin: J.A. Sennello, et al.; Endocrinology 146, 2157 (2005) Abstract
General Information

Background/Technical Information   

Swiss-Prot link Q60994: Adiponectin (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

GENERAL LITERATURE REFERENCES
Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30): T.S. Tsao, et al.; J. Biol. Chem. 277, 29359 (2002) Full Text; http://www.jbc.org/cgi/content/full/277/33/29359
Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity: U.B. Pajvani, et al.; J. Biol. Chem. 279, 12152 (2004) Full Text; http://www.jbc.org/cgi/reprint/279/13/12152
RELATED PRODUCT GROUPS   

Proteins > Recombinant Proteins / Fusion Proteins