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ALX-522-063 [ALEXIS] Revised 28-Aug-07
Adiponectin (human) (recombinant) SYNONYMS    ACRP30 (human) (recombinant) Adipocyte Complement Related Protein of 30kDa (human) (recombinant) AdipoQ (human) (recombinant) apM1 (human) (recombinant) GBP28 (human) (recombinant) PRODUCT LINE    Obesity & Adipokines PRODUCT FAMILIES    Adiponectin [ACRP30] / Related Products Ordering Information  Product Numbers Format Size Unit Price Quantity  ALX-522-063-C050 50 µg     Product Specification SPECIES CROSSREACTIVITY:    Human   MW:    ~33kDa (SDS-PAGE).   SOURCE/HOST:    Produced in HEK 293 cells. Human adiponectin (aa 16-244) is fused at the N-terminus to a linker peptide (14 aa) and a FLAG®-tag.   CONCENTRATION:    1mg/ml after reconstitution.   PURITY:    ≥90% (SDS-PAGE)   FORMULATION:    Lyophilized. Contains PBS.   ENDOTOXIN CONTENT:    <0.1EU/µg purified protein (LAL test; Bio Whittaker).   RECONSTITUTION:    Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein.   BIOLOGICAL ACTIVITY:    Activates AMPK.   SHIPPING:    SHIPPED ON BLUE ICE   LONG TERM STORAGE:    -20°C   USE/STABILITY:    Stable for at least 6 months after receipt when stored at -20°C.   HANDLING:    Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. Product Description Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species. Product Specific Literature References Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase: A. Suzuki, et al.; Mol. Cell. Biol. 27, 4317 (2007) Abstract General Information Background/Technical Information    Swiss-Prot link Q15848: Adiponectin (human) (precursor) FLAG is a registered trademark of Sigma-Aldrich Co. GENERAL LITERATURE REFERENCES ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism: A.H. Berg, et al.; Trends Endocrinol. Metab. 13, 84 (2002), (Review) Abstract; Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30): T.S. Tsao, et al.; J. Biol. Chem. 277, 29359 (2002) Full Text; http://www.jbc.org/cgi/content/full/277/33/29359 Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity: U.B. Pajvani, et al.; J. Biol. Chem. 279, 12152 (2004) Full Text; http://www.jbc.org/cgi/reprint/279/13/12152 RELATED PRODUCT GROUPS    Proteins > Recombinant Proteins / Fusion Proteins