Adiponectin (Globular Domain) (mouse) (recombinant) |
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| SYNONYMS | ACRP30 (Globular Domain) (mouse) (recombinant)
Adipocyte Complement Related Protein of 30kDa (Globular Domain) (mouse) (recombinant)
AdipoQ (Globular Domain) (mouse) (recombinant)
apM1 (Globular Domain) (mouse) (recombinant)
GBP28 (Globular Domain) (mouse) (recombinant) |
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| PRODUCT LINE | Obesity & Adipokines |
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| PRODUCT FAMILIES | Adiponectin [ACRP30] / Related Products |
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| Ordering Information |
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| Product Numbers | Format | Size | Unit Price | Quantity | | | ALX-522-088-C050 |
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50 µg | | | | | | |
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| Product Specification |
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| MW: | ~26kDa |
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| SOURCE/HOST: | Produced in HEK 293 cells. The globular domain of mouse adiponectin (aa 111-247) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
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| CONCENTRATION: | 1mg/ml after reconstitution. |
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| PURITY: | >95% (SDS-PAGE) |
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| FORMULATION: | Lyophilized. Contains PBS. |
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| ENDOTOXIN CONTENT: | <0.1EU/µg protein (LAL test; BioWhittaker). |
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| RECONSTITUTION: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| SHIPPING: | SHIPPED ON BLUE ICE |
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| LONG TERM STORAGE: | -20°C |
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| USE/STABILITY: | Stable for at least 6 months after receipt when stored at -20°C. |
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| HANDLING: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| | | General Information | | Adiponectin, also known as ACRP30 (30kDa adipocyte complement-related protein) is a hormone synthesized and secreted by adipocytes. Expression and plasma levels of adiponectin are decreased in insulin resistance and obesity and synthesis of this adipokine increases when insulin sensitivity is improved and weight is lost.
Adiponectin consists of a carboxyl-terminal globular domain and an amino-terminal collagen-like domain containing 22 Gly-X-Y repeats. The presence of a small amount of a carboxyl-terminal fragment containing the globular domain of adiponectin (gAdiponectin) has been demonstrated in human serum by immunoprecipitation. Adiponectin and gAdiponectin act in the brain to increase energy expenditure and to decrease body weight. |
Background/Technical Information | | FLAG is a registered trademark of Sigma-Aldrich Co. | | | | GENERAL LITERATURE REFERENCES |
Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice: J. Fruebis, et al.; PNAS 98, 2005 (2001) Full Text; http://www.pnas.org/cgi/reprint/98/4/2005
Adiponectin, obesity, and cardiovascular disease: M. Fasshauer, et al.; Biochimie 86, 779 (2004) Abstract;
Adiponectin acts in the brain to decrease body weight: Y. Qi, et al.; Nat. Med. 10, 524 (2004) Abstract;
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| RELATED PRODUCT GROUPS |
Proteins > Recombinant Proteins / Fusion Proteins |
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